Journal: Molecular & Cellular Proteomics : MCP

Article Title: Fasciola hepatica Surface Tegument: Glycoproteins at the Interface of Parasite and Host *

doi: 10.1074/mcp.M116.059774

Figure Lengend Snippet: Lectin microarray reveals that Fasciola hepatica tegument (FhTeg) exhibits a rich and complex oligosaccharide make up. Fluorescently labeled FhTeg (8.6 μg ml −1 ) was incubated with a proprietary lectin microarray (1 h, 23 °C, 4 rpm) in TBS-T supplemented with 1 m m Ca 2+ and Mg 2+ . Slides were scanned in a microarray scanner using a 543 nm laser. Histogram represents the the mean fluorescence intensity of three experimental replicates (18 data points in total) of fluorescently labeled FhTeg binding to printed lectins. Error bars are the standard deviation of the mean of three experimental replicates ( A ). Binding data is also represented as a heat map and depicts both mean intensity and individual replicates ( B ) in which little to no binding intensity is represented by green and greatest intensity by red.

Article Snippet: A panel of 48 lectins ( supplemental Table S1 ) was printed on Nexterion® Slide H microarray slides in a 62% (±2%) humidity environment using a SciFLEXARRAYER S3 (Scienion AG, Berlin, Germany) equipped with a 90 μm uncoated glass dispenser capillary to construct the lectin microarray as previously described ( ).

Techniques: Microarray, Labeling, Incubation, Fluorescence, Binding Assay, Standard Deviation

Journal: International Journal of Molecular Sciences

Article Title: Glycosylation and Characterization of Human Transferrin in an End-Stage Kidney Disease

doi: 10.3390/ijms25094625

Figure Lengend Snippet: Lectins used in microarray; their glycan specificity and reactivity with a PD transferrin (weak “+” and strong “++”).

Article Snippet: A microarray analysis was performed with each sample separately using biotinylated lectins, epoxy microarray slides (NEXTERION Slide E, Schott, Germany), a non-contact piezoelectric sciFLEXARRAYER S1 microarray spotter and a piezo dispense capillary PDC 80 (Scienion AG, Berlin, Germany) [ ].

Techniques: Microarray, Glycoproteomics, Whole Genome Amplification

Journal: International Journal of Molecular Sciences

Article Title: Glycosylation and Characterization of Human Transferrin in an End-Stage Kidney Disease

doi: 10.3390/ijms25094625

Figure Lengend Snippet: Reactivity of transferrin isolated from healthy persons (H) and patients on peritoneal dialysis (PD) with lectins. A statistically significant difference between groups ( p < 0.05) is labeled with “*”.

Article Snippet: A microarray analysis was performed with each sample separately using biotinylated lectins, epoxy microarray slides (NEXTERION Slide E, Schott, Germany), a non-contact piezoelectric sciFLEXARRAYER S1 microarray spotter and a piezo dispense capillary PDC 80 (Scienion AG, Berlin, Germany) [ ].

Techniques: Isolation, Labeling